Molecular Genetic and Serologic Analysis of the O allele in the Korean Population / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 124-137, 2019.
Artículo
en Inglés
| WPRIM
| ID: wpr-759593
ABSTRACT
BACKGROUND:
The recent expansion of knowledge about various ABO alleles has led to the need for a comprehensive measure to cover the numerous polymorphisms dispersed in the ABO gene. A few studies have examined the diversity of the O allele compared to A or B subgroup alleles, resulting in antigenic changes. This study investigated the relationship between the serologic and molecular genetic characteristics of the O alleles in the Korean population.METHODS:
One hundred and five samples from healthy blood group O subjects were selected randomly. The isoagglutinin titer was measured using a tube agglutination and gel microcolumn assay. The ABO alleles were analyzed by sequencing exons 6 and 7 of the ABO gene. When the origin of a heterozygous nucleotide sequence was ambiguous, it was separated into a single allele using mono-allele amplification or cloning.RESULTS:
The median IgM isoagglutinin titer was eight. In contrast, the median IgG anti-A and anti-B isoagglutinin titers were 64 and 32, respectively. The IgG isoagglutinin titer showed a significant increase with age (P<0.0001). Six O alleles were observed in 105 blood group O populations by sequencing. The O01 and O02 alleles were common (0.57, 0.36). Three rare O alleles (O04, O05, and O06) and one novel non-deletional O allele were found.CONCLUSION:
The distribution of isoagglutinin titers of blood group O and the genetic frequency of O alleles in this study would form the basis of the development and interpretation of ABO genotyping and serologic workup in the Korean population.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Inmunoglobulina G
/
Inmunoglobulina M
/
Secuencia de Bases
/
Exones
/
Células Clonales
/
Análisis de Secuencia
/
Clonación de Organismos
/
Aglutinación
/
Alelos
/
Biología Molecular
Idioma:
Inglés
Revista:
Korean Journal of Blood Transfusion
Año:
2019
Tipo del documento:
Artículo
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