Astaxanthin Prevents Decreases in Superoxide Dismutase 2 Level and Superoxide Dismutase Activity in Helicobacter pylori-infected Gastric Epithelial Cells
Journal of Cancer Prevention
;
: 54-58, 2019.
Artículo
en Inglés
| WPRIM
| ID: wpr-764294
ABSTRACT
BACKGROUND:
Helicobacter pylori increases production of reactive oxygen species (ROS), which activates inflammatory and carcinogenesis-related signaling pathways in gastric epithelial cells. Therefore, reducing ROS, by upregulating antioxidant enzyme, such as superoxide dismutase (SOD), may be a novel strategy to prevent H. pylori-associated gastric diseases. Astaxanthin is an antioxidant carotenoid that prevents oxidative stress-induced cell injury. The present study was aimed to determine whether H. pylori decreases SOD activity by changing the levels of SOD1/SOD2 and whether astaxanthin prevents changes in SOD levels and activity in H. pylori-infected gastric epithelial AGS cells.METHODS:
AGS cells were pre-treated with astaxanthin for 3 hours prior to H. pylori infection and cultured for 1 hour in the presence of H. pylori. SOD levels and activity were assessed by Western blot analysis and a commercial assay kit, respectively. Mitochondrial ROS was determined using MitoSOX fluorescence.RESULTS:
H. pylori decreased SOD activity and the SOD2 level, but increased mitochondrial ROS in AGS cells. The SOD1 level was not changed by H. pylori infection. Astaxanthin prevented H. pylori-induced decreases in the SOD2 level and SOD activity and reduced mitochondrial ROS in AGS cells.CONCLUSIONS:
Consumption of astaxanthin-rich food may prevent the development of H. pylori-associated gastric disorders by suppressing mitochondrial oxidative stress.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Gastropatías
/
Superóxido Dismutasa
/
Western Blotting
/
Helicobacter pylori
/
Helicobacter
/
Especies Reactivas de Oxígeno
/
Superóxidos
/
Estrés Oxidativo
/
Células Epiteliales
/
Fluorescencia
Idioma:
Inglés
Revista:
Journal of Cancer Prevention
Año:
2019
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS