β-carotene Inhibits Expression of c-Myc and Cyclin E in Helicobacter pylori-infected Gastric Epithelial Cells
Journal of Cancer Prevention
;
: 192-196, 2019.
Artículo
en Inglés
| WPRIM
| ID: wpr-764309
ABSTRACT
BACKGROUND:
Helicobacter pylori infection is a major risk factor in the development of gastric cancer. H. pylori infection of gastric epithelial cells increases the levels of reactive oxygen species (ROS), activates oncogenes, and leads to β-catenin-mediated hyper-proliferation. β-Carotene reduces ROS levels, inhibits oxidant-mediated activation of inflammatory signaling and exhibits anticancer properties. The present study was carried out to determine if β-carotene inhibits H. pylori-induced cell proliferation and the expression of oncogenes c-myc and cyclin E by reducing the levels of β-catenin and phosphorylated glycogen synthase kinase 3β (p-GSK3β).METHODS:
Gastric epithelial AGS cells were pre-treated with β-carotene (5 and 10 μM) for 2 hours prior to H. pylori infection and cultured for 6 hours (for determination of the levels of p-GSK3β, GSK3β, and β-catenin) and 24 hours (for determination of cell viability and protein levels of c-myc and cyclin E). Cell viability was determined by the MTT assay and protein levels were determined via western blot-based analysis.RESULTS:
β-Carotene inhibited H. pylori-induced increases in the percentage of viable cells, phosphorylated GSK3β (p-GSK3β), and the levels of β-catenin, c-myc and cyclin E.CONCLUSIONS:
β-Carotene inhibits H. pylori-induced hyper-proliferation of gastric epithelial cells by suppressing β-catenin signaling and oncogene expression.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oncogenes
/
Neoplasias Gástricas
/
Supervivencia Celular
/
Factores de Riesgo
/
Helicobacter pylori
/
Helicobacter
/
Especies Reactivas de Oxígeno
/
Ciclinas
/
Beta Caroteno
/
Ciclina E
Tipo de estudio:
Estudio de etiología
/
Factores de riesgo
Idioma:
Inglés
Revista:
Journal of Cancer Prevention
Año:
2019
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS