Changes in the composition of artificial cariogenic biofilms over time / 대한구강보건학회지
Journal of Korean Academy of Oral Health
;
: 118-123, 2019.
Artículo
en Coreano
| WPRIM
| ID: wpr-764721
ABSTRACT
OBJECTIVES:
The purpose of this study was to investigate changes in the composition of artificial cariogenic biofilms using a Streptococcus mutans biofilm model over a period of time.METHODS:
We analyzed the dry weight, colony forming unit (CFU) number, extracellular polysaccharide (EPS) biovolume, and acid production rate of S. mutans biofilms formed on saliva-coated hydroxyapatite discs after 26 h, 50 h, 74 h, 98 h, 171 h, and 195 h. In addition, we performed a laser scanning confocal fluorescence microscopy to determine the bacterial volume, EPS biovolume, and biofilm thickness. We calculated the biofilm density using dry weight and EPS biovolume.RESULTS:
Over a period of time, there was no change in the CFU number and acid production rate of S. mutans biofilms, but there was an increase in the dry weight and EPS biovolume of S. mutans biofilms. The bacterial volume, EPS biovolume, and biofilm thickness only increased in the 50-h-old biofilm; however, no change was observed in 50-195-h-old biofilms. In addition, an increase in the biofilm density was observed over time.CONCLUSIONS:
These results suggest that the acid production ability of cariogenic biofilms does not change, but the biofilm density increases over time. However, due to scientific information, further research needs to be conducted in the field of dentistry to get further insights on the progression of cariogenic biofilms over time.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Madre
/
Streptococcus mutans
/
Durapatita
/
Biopelículas
/
Odontología
/
Microscopía Fluorescente
Idioma:
Coreano
Revista:
Journal of Korean Academy of Oral Health
Año:
2019
Tipo del documento:
Artículo
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