Methods for mammalian single cell research - a review / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 27-39, 2019.
Artículo
en Chino
| WPRIM
| ID: wpr-771403
ABSTRACT
Basic research in life science and medicine has dug into single cell level in recent years. Single-cell analysis offers to understand life from diverse perspectives and is used to profile cell heterogeneity to investigate mechanism of diseases. Single cell technologies have also found applications in forensic medicine and clinical reproductive medicine, while the techniques are rapidly evolving and have become more and more sophisticated. In this article, we reviewed various single cell isolation techniques and their pros and cons, including manual cell picking, laser capture microdissection and microfluidics, as well as analysis methods for DNA, RNA and protein in single cell. In addition, we summarized major up-to-date single cell research achievements and their potential applications.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN
/
ARN
/
Separación Celular
/
Análisis de la Célula Individual
/
Captura por Microdisección con Láser
Tipo de estudio:
Guía de Práctica Clínica
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2019
Tipo del documento:
Artículo
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