Preliminary study of determination of Shougong in Jinlong Capsules based on fluorescence quantitative PCR method / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4582-4586, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-771546
ABSTRACT
A pair of species-specific primer (GZG1/GZG2) based on COⅠ sequence regions for identification of Gekko chinensis were designed. A fluorescent quantitative PCR method was established to identify and quantify G. chinensis from Jinlong Capsules Formula. A standard curve for quantitative analysis of G. chinensis was established (the standard curve equation y=-3.012 7x+34.501, y is Ct value, x is lg N, N is the copies of COⅠ fragment from G. chinensis). Samples included G. chinensis appeared amplification, while falsify group (not included G. chinensis) and negative control did not have amplification products. The copy number of COⅠ region of G. chinensis was respectively 11.511×10⁶, 6.416×10⁶, 2.553×10⁶ copies/μL in all quality goods, quality goods-adulterants 11, quality goods-adulterants 14. The results accorded with proportion of adding amount roughly. This study can provide a new strategy for quality control of Chinese patent medicine containing animal drug ingredients.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cápsulas
/
Medicamentos Herbarios Chinos
/
Reacción en Cadena de la Polimerasa
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2018
Tipo del documento:
Artículo
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