A Study on the Proteins that Interact with Human Nebulin SH3 Domain / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 133-139, 2002.
Artículo
en Coreano
| WPRIM
| ID: wpr-77163
ABSTRACT
OBJECTIVE:
bjective By identifying the unknown substance responsible for binding with nebulin SH3 domain within the sarcomeric Z-line, we tried to find out Z-line structure which plays an important role on muscle contraction and maintenance of muscle funtion.METHOD:
First, the bait plasmid was made by binding the DNA binding domain of Gal4 protein of yeast and the SH3 domain. Second, library plasmid was made by binding activation domain and human skeletal cDNA library. Then, the base sequence of the clone, produced by combining the two proteins expressed by transgenically converted plasmid in yeast, was analyzed.RESULT:
We screened out six true positive clones and analyzed the base sequence of the two of six clones. We identified them to be alpha-actinin2.CONCLUSION:
We can theorize that Neublin SH3 domain and alpha-actinin2 plays a vital role for the integration of Z-line. Thus, this is an important data in further studying muscle functions, mechanisms, and muscular disease as well.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
Levaduras
/
ADN
/
Secuencia de Bases
/
Biblioteca de Genes
/
Células Clonales
/
Dominios Homologos src
/
Contracción Muscular
/
Enfermedades Musculares
Límite:
Humanos
Idioma:
Coreano
Revista:
Journal of Korean Orthopaedic Research Society
Año:
2002
Tipo del documento:
Artículo
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