Imprinting genes modified parthenogenetic embryonic stem cells produce full-term mouse via tetraploid complementation / 生物工程学报
Chinese Journal of Biotechnology
; (12): 910-918, 2019.
Article
en Zh
| WPRIM
| ID: wpr-771835
Biblioteca responsable:
WPRO
ABSTRACT
Parthenogenetic embryonic stem cells (pESCs) derived from bi-maternal genomes do not have competency of tetraploid complementation, due to lacking of paternal imprinting genes. To make pESCs possess fully development potentials and similar pluripotency to zygote-derived ESCs, we knocked out one allelic gene of the two essential maternal imprinting genes (H19 and IG) in their differentially methylated regions (DMR) via CRISPR/Cas9 system and obtained double knock out (DKO) pESCs. Maternal pESCs had similar morphology, expression levels of pluripotent makers and in vitro neural differentiation potentials to zygotes-derived ESCs. Besides that, DKO pESCs could contribute to full-term fetuses through tetraploid complementation, proving that they held fully development potentials. Derivation of DKO pESCs provided a type of major histocompatibility complex (MHC) matched pluripotent stem cells, which would benefit research in regenerative medicine.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Partenogénesis
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Impresión Genómica
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Células Madre Pluripotentes
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Medicina Regenerativa
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Células Madre Embrionarias
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Técnicas de Inactivación de Genes
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Tetraploidía
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2019
Tipo del documento:
Article