Application and Evaluation of Chemiluminescence Immunoassay in Blood Screening / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 569-572, 2019.
Artículo
en Chino
| WPRIM
| ID: wpr-771918
ABSTRACT
OBJECTIVE@#To explore the application of blood screening method based on chemiluminescence immunoassay(CLIA)and to evaluate its officacy.@*METHODS@#Screening HBsAg, anti-HCV, anti-HIV and TP was performed on 3,530 voluntary blood donors by ELISA and CLIA, and then all the specimens with ELISA and ELISA/CLIA were further confirmed by NAT; TP single and double positive specimens by ELISA or CLIA were further confirmed by TPPA.@*RESULTS@#The results of CLIA method was well consistent with NAT results, displaying better repeatability and higher sensitivity than ELISA method. For CLIA/ELISA specimens there was a certain false-negative result obtained by ELISA method, especially for blood donors with low virus biter concentration or "window period".@*CONCLUSION@#ELISA and CLIA have complementary advantages in blood screening, which can improve the sensitivity of blood screening, reduce the missed detection and shorten detection time. The introduction of CLIA for blood screening is of great importance for ensuring the quality of blood and the safety of clinical transfusion.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Donantes de Sangre
/
Virus de la Hepatitis B
/
Tamizaje Masivo
/
Luminiscencia
/
Antígenos de Superficie de la Hepatitis B
/
Mediciones Luminiscentes
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2019
Tipo del documento:
Artículo
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