TLR/NF-κB independent signaling pathway in TNF-α suppression of diabetic MyD88-knockout mice after polysaccharides administration / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 35-40, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-772601
ABSTRACT
OBJECTIVE@# To investigate the effect of polysaccharides (LBPs) on TLR/NF-κB independent pathway and serum tumor necrosis factor (TNF-α) level in diabetic MyD88-knockout mice.@*METHODS@# Diabetes was induced by feeding high-fat/high-sugar diet and injection of low-dose streptozotocin in MyD88-knockout mice. The diabetic mice were randomly divided into model group, positive control group and LBPs group. The expressions of TRAM, TRIF, TRAF6, RIP1 and TNF-α mRNA and proteins in mouse peritoneal macrophages were detected by real-time RT-PCR and Western blotting after LBPs treatment for 3 month. Serum TNF-α was determined with ELISA kit.@*RESULTS@# Real time RT-PCR showed that compared with model group, the relative expressions of and mRNA in macrophages of LBPs group were significantly decreased and expression of was significantly increased (all 0.05).@*CONCLUSIONS@# LBPs may not inhibit serum TNF-α level through TLR/NF-κB independent pathway.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Sangre
/
Medicamentos Herbarios Chinos
/
Transducción de Señal
/
Distribución Aleatoria
/
Regulación de la Expresión Génica
/
FN-kappa B
/
Factor de Necrosis Tumoral alfa
/
Ratones Noqueados
/
Macrófagos Peritoneales
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2018
Tipo del documento:
Artículo
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