Isolation and identification of endophytic fungi producing harpagoside and harpagide from Scrophularia ningpoensis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2046-2050, 2019.
Artículo
en Chino
| WPRIM
| ID: wpr-773130
ABSTRACT
The endophytic fungi from root,main stem,branch and leaf of Scrophularia ningpoensis were isolated from Zhejiang,whether these strains could yield harpagide or harpagoside were tested by HPLC and LC-MS. According to the morphological characteristic and the similarity of the nucleotide sequence of internal transcribed spacer( ITS) between r DNAs,the strains producing harpagide or harpagoside were identified. The results showed that 210 strains were isolated from the samples,which were classified into 9 orders,13 families and 17 genera by morphological study. Harpagide was detected in endogenous fungi ZJ17 and harpagoside was detected in endogenous fungi ZJ25 by HPLC coupled with LC-MS. ZJ17 was identified as Alternaria alternate and ZJ25 was identified as A.gaisen by its morphology and authenticated by ITS( ITS4 and ITS5 regions and the intervening 5. 8 S rDNA region).
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Piranos
/
ADN de Hongos
/
China
/
Clasificación
/
ADN Espaciador Ribosómico
/
Scrophularia
/
Glicósidos Iridoides
/
Endófitos
/
Hongos
/
Genética
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2019
Tipo del documento:
Artículo
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