Reversal of multidrug resistance by icaritin in doxorubicin-resistant human osteosarcoma cells / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 20-28, 2018.
Article
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| ID: wpr-773642
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WPRO
ABSTRACT
Multidrug resistance (MDR) is one of the major obstacles in cancer chemotherapy. Our previous study has shown that icariin could reverse MDR in MG-63 doxorubicin-resistant (MG-63/DOX) cells. It is reported that icariin is usually metabolized to icariside II and icaritin. Herein, we investigated the effects of icariin, icariside II, and icaritin (ICT) on reversing MDR in MG-63/DOX cells. Among these compounds, ICT exhibited strongest effect and showed no obvious cytotoxicity effect on both MG-63 and MG-63/DOX cells ranging from 1 to 10 μmol·L. Furthermore, ICT increased accumulation of rhodamine 123 and 6-carboxyfluorescein diacetate and enhanced DOX-induced apoptosis in MG-63/DOX cells in a dose-dependent manner. Further studies demonstrated that ICT decreased the mRNA and protein levels of multidrug resistance protein 1 (MDR1) and multidrug resistance-associated protein 1 (MRP1). We also verified that blockade of STAT3 phosphorylation was involved in the reversal effect of multidrug resistance in MG-63/DOX cells. Taken together, these results indicated that ICT may be a potential candidate in chemotherapy for osteosarcoma.
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Índice:
WPRIM
Asunto principal:
Patología
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Farmacología
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Fosforilación
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Triterpenos
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Flavonoides
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Doxorrubicina
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Osteosarcoma
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Regulación Neoplásica de la Expresión Génica
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Supervivencia Celular
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Apoptosis
Límite:
Humans
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Año:
2018
Tipo del documento:
Article