Establishment of cell lines for quality control of prenatal genetic diagnosis by gene transfection / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 520-524, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-775284
ABSTRACT
OBJECTIVE@#To establish a cell lines for quality control of prenatal genetic diagnosis.@*METHODS@#The recombined SV40LTag-pcDNA3.1(-) vector was constructed and transfected by lipidosome into human amniotic fluid cells with common aneuploidy. Positive clones were screened by G418, and the immortality of transfected cell line was identified.@*RESULTS@#Cell line with karyotype of 46, XY, t(8;19)(q24.3;q13.1) from primary amniotic fluid cells was established. Karyotype analytical results indicated that the cell line at its 15th generation maintained the same karyotype of its primary cell.@*CONCLUSIONS@#Gene can lead to immortality of amniotic fluid cells, which contributes to preparing cell lines for internal and external quality control in prenatal genetic diagnosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Diagnóstico Prenatal
/
Control de Calidad
/
Proteínas Recombinantes
/
Transfección
/
Línea Celular
/
Antígenos Transformadores de Poliomavirus
/
Cariotipo
/
Vectores Genéticos
/
Genética
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Límite:
Femenino
/
Humanos
/
Embarazo
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2018
Tipo del documento:
Artículo
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