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Establishment of cell lines for quality control of prenatal genetic diagnosis by gene transfection / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences ; (6): 520-524, 2018.
Artículo en Chino | WPRIM | ID: wpr-775284
ABSTRACT
OBJECTIVE@#To establish a cell lines for quality control of prenatal genetic diagnosis.@*METHODS@#The recombined SV40LTag-pcDNA3.1(-) vector was constructed and transfected by lipidosome into human amniotic fluid cells with common aneuploidy. Positive clones were screened by G418, and the immortality of transfected cell line was identified.@*RESULTS@#Cell line with karyotype of 46, XY, t(8;19)(q24.3;q13.1) from primary amniotic fluid cells was established. Karyotype analytical results indicated that the cell line at its 15th generation maintained the same karyotype of its primary cell.@*CONCLUSIONS@#Gene can lead to immortality of amniotic fluid cells, which contributes to preparing cell lines for internal and external quality control in prenatal genetic diagnosis.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Diagnóstico Prenatal / Control de Calidad / Proteínas Recombinantes / Transfección / Línea Celular / Antígenos Transformadores de Poliomavirus / Cariotipo / Vectores Genéticos / Genética / Métodos Tipo de estudio: Estudio diagnóstico Límite: Femenino / Humanos / Embarazo Idioma: Chino Revista: Journal of Zhejiang University. Medical sciences Año: 2018 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Diagnóstico Prenatal / Control de Calidad / Proteínas Recombinantes / Transfección / Línea Celular / Antígenos Transformadores de Poliomavirus / Cariotipo / Vectores Genéticos / Genética / Métodos Tipo de estudio: Estudio diagnóstico Límite: Femenino / Humanos / Embarazo Idioma: Chino Revista: Journal of Zhejiang University. Medical sciences Año: 2018 Tipo del documento: Artículo