Determination of impurity diketo aldehyde in bulk drug and preparations of dihydroartemisinin / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4069-4073, 2018.
Article
en Zh
| WPRIM
| ID: wpr-775377
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WPRO
ABSTRACT
Diketo aldehyde (DKA),one of the most important impurities in dihydroartemisinin,was synthesized through reaction between dihydroartemisinin and anhydrous ferrous bromide under a N₂ atmosphere, and an HPLC method was established for the determination of DKA in bulk drug and in DHA tablet. DKA was prepared from dihydroartemisinin in the presence of FeBr₂.The chromatographic separation was achieved through an Agilent Eclise XDB-C₁₈ column (4.6 mm×250 mm,5 μm), and the optimal mobile phase consisted of acetonitrile and water in the ratio of 37:63 at flow rate of 1.0 mL·min⁻¹.The detection was carried out at 216 nm, and column temperature was 15 °C.The injection volume was 40 μL.The method featured a good linearity (=0.999 9),precision (1.0%),repeatability (1.3%),stability (DKA standards RSD=1.0% and in tablet form instability),recovery (92.88%),limits of detection (0.20 mg·L⁻¹) ,and limits of quantification (0.78 mg·L⁻¹). The result show that the content of DKA in bulk drug was 0.086 7%-2.622 9%, and the content of DKA in tablet was 0.068 3%-0.615 1%.
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Asunto principal:
Estándares de Referencia
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Contaminación de Medicamentos
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Cromatografía Líquida de Alta Presión
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Artemisininas
/
Aldehídos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2018
Tipo del documento:
Article