Neuroligins Differentially Mediate Subtype-Specific Synapse Formation in Pyramidal Neurons and Interneurons / 神经科学通报·英文版
Neuroscience Bulletin
;
(6): 497-506, 2019.
Artículo
en Inglés
| WPRIM
| ID: wpr-775419
ABSTRACT
Neuroligins (NLs) are postsynaptic cell-adhesion proteins that play important roles in synapse formation and the excitatory-inhibitory balance. They have been associated with autism in both human genetic and animal model studies, and affect synaptic connections and synaptic plasticity in several brain regions. Yet current research mainly focuses on pyramidal neurons, while the function of NLs in interneurons remains to be understood. To explore the functional difference among NLs in the subtype-specific synapse formation of both pyramidal neurons and interneurons, we performed viral-mediated shRNA knockdown of NLs in cultured rat cortical neurons and examined the synapses in the two major types of neurons. Our results showed that in both types of neurons, NL1 and NL3 were involved in excitatory synapse formation, and NL2 in GABAergic synapse formation. Interestingly, NL1 affected GABAergic synapse formation more specifically than NL3, and NL2 affected excitatory synapse density preferentially in pyramidal neurons. In summary, our results demonstrated that different NLs play distinct roles in regulating the development and balance of excitatory and inhibitory synapses in pyramidal neurons and interneurons.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Sinapsis
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Moléculas de Adhesión Celular Neuronal
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Células Cultivadas
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Corteza Cerebral
/
Embriología
/
Ratas Sprague-Dawley
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Células Piramidales
/
Isoformas de Proteínas
/
Neuronas GABAérgicas
Límite:
Animales
Idioma:
Inglés
Revista:
Neuroscience Bulletin
Año:
2019
Tipo del documento:
Artículo
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