Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin / 神经科学通报·英文版
Neuroscience Bulletin
;
(6): 205-215, 2019.
Artículo
en Inglés
| WPRIM
| ID: wpr-775425
ABSTRACT
The locus coeruleus (LC) has been studied in major depressive disorder (MDD) and bipolar disorder (BD). A major problem of immunocytochemical studies in the human LC is interference with the staining of the immunocytochemical end-product by the omnipresent natural brown pigment neuromelanin. Here, we used a multispectral method to untangle the two colors blue immunocytochemical staining and brown neuromelanin. We found significantly increased tyrosine hydroxylase (TH) in the LC of MDD patients-thus validating the method-but not in BD patients, and we did not find significant changes in the receptor tyrosine-protein kinase ErbB4 in the LC in MDD or BD patients. We observed clear co-localization of ErbB4, TH, and neuromelanin in the LC neurons. The different stress-related molecular changes in the LC may contribute to the different clinical symptoms in MDD and BD.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Análisis Espectral
/
Tirosina 3-Monooxigenasa
/
Trastorno Bipolar
/
Procesamiento de Imagen Asistido por Computador
/
Locus Coeruleus
/
Inmunohistoquímica
/
Sensibilidad y Especificidad
/
Trastorno Depresivo Mayor
/
Receptor ErbB-4
Tipo de estudio:
Estudio diagnóstico
Límite:
Anciano
/
Aged80
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Neuroscience Bulletin
Año:
2019
Tipo del documento:
Artículo
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