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Optimization of chromosome flaking technique for mesenchymal stem cells / 中华医学遗传学杂志
Chinese Journal of Medical Genetics ; (6): 143-146, 2019.
Artículo en Chino | WPRIM | ID: wpr-775793
ABSTRACT
OBJECTIVE@#To optimize the condition for chromosome flaking of mesenchymal stem cells to ensure the cytogenetic quality control of expanding production and clinical application.@*METHODS@#Chromosomal flaking methods were optimized from current chromosome preparation techniques from the aspects of MSCs cell culture concentration, colchicine treatment time and low permeability time.@*RESULTS@#By repeated pre-experiments, the optimal MSCS chromosome flaking condition of MSCs was determined as cell culture concentration of (1-2)× 10 cells per T25 cell culture bottle, and the colchicines processing time was determined as 2 hours and 10 minutes, and the low permeability was 1 hour.@*CONCLUSION@#The optimized chromosome flaking condition can fulfill the requirement of cytogenetic quality control for MSCs.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Diferenciación Celular / Células Cultivadas / Cromosomas Humanos / Técnicas de Cultivo de Célula / Trastornos de los Cromosomas / Citogenética / Células Madre Mesenquimatosas Límite: Humanos Idioma: Chino Revista: Chinese Journal of Medical Genetics Año: 2019 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Diferenciación Celular / Células Cultivadas / Cromosomas Humanos / Técnicas de Cultivo de Célula / Trastornos de los Cromosomas / Citogenética / Células Madre Mesenquimatosas Límite: Humanos Idioma: Chino Revista: Chinese Journal of Medical Genetics Año: 2019 Tipo del documento: Artículo