Optimization of chromosome flaking technique for mesenchymal stem cells / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 143-146, 2019.
Artículo
en Chino
| WPRIM
| ID: wpr-775793
ABSTRACT
OBJECTIVE@#To optimize the condition for chromosome flaking of mesenchymal stem cells to ensure the cytogenetic quality control of expanding production and clinical application.@*METHODS@#Chromosomal flaking methods were optimized from current chromosome preparation techniques from the aspects of MSCs cell culture concentration, colchicine treatment time and low permeability time.@*RESULTS@#By repeated pre-experiments, the optimal MSCS chromosome flaking condition of MSCs was determined as cell culture concentration of (1-2)× 10 cells per T25 cell culture bottle, and the colchicines processing time was determined as 2 hours and 10 minutes, and the low permeability was 1 hour.@*CONCLUSION@#The optimized chromosome flaking condition can fulfill the requirement of cytogenetic quality control for MSCs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Diferenciación Celular
/
Células Cultivadas
/
Cromosomas Humanos
/
Técnicas de Cultivo de Célula
/
Trastornos de los Cromosomas
/
Citogenética
/
Células Madre Mesenquimatosas
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2019
Tipo del documento:
Artículo
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