Construction of a glucaric acid biosensor for screening myo-inositol oxygenase variants / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1772-1783, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-776291
ABSTRACT
Glucaric acid (GA), a top value-added chemical from biomass, has been widely used for prevention and control of diseases and the production of polymer materials. In GA biosynthesis pathway, the conversion of inositol to glucuronic acid that catalyzed by myo-inositol oxygenase is the limiting step. It is necessary to improve MIOX activity. In the present study, we constructed a high-throughput screening system through combing the concentration of GA with the green fluorescent protein fluorescence intensity. By applying this screening system, three positive variants (K59V/R60A, R171S and D276A) screened from the mutant library. In comparison, the recombinant strain Escherichia coli BL21(DE3)/MU-R171S accumulated more GA, 136.5% of that of the parent strain.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Técnicas Biosensibles
/
Química
/
Escherichia coli
/
Inositol-Oxigenasa
/
Vías Biosintéticas
/
Ácido Glucárico
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2018
Tipo del documento:
Artículo
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