Identification of chemical constituents in Sinopodophylli Fructus by HPLC-DAD-ESI-IT-TOF-MSn / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 123-133, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-776413
ABSTRACT
This experiment was performed to analyze and identify the chemical constituents of Sinopodophylli Fructus by HPLC-DAD-ESI-IT-TOF-MSn. The analysis was performed on an Agilent Zorbax SB-C₁₈ (4.6 mm×250 mm, 5 μm) column.The mobile phase consisted of 0.1% formic acid was used for gradient at a flow rate of 1.0 mL·min⁻¹. Electrospray ionization ion trap time-of-flight multistage mass spectrometry was applied for qualitative analysis under positive and negative ion modes. The results indicated that 54 compounds consisted of 18 lignans and 36 flavonoids from Xiaoyelian had been detected by their HRMS data, the information of literature and reference substance. Among them, 27 compounds were reported in Sinopodophylli Fructus for the first time. In conclusion, an HPLC-DAD-ESI-IT-TOF-MSn method was established to qualitative analysis of Xiaoyelian in this study, which will provide the evidence for evaluating the quality of Xiaoyelian herbs, clarifying the mechanism, and guiding the development of pharmacological active ingredients.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Flavonoides
/
Medicamentos Herbarios Chinos
/
Química
/
Cromatografía Líquida de Alta Presión
/
Lignanos
/
Espectrometría de Masa por Ionización de Electrospray
/
Berberidaceae
/
Espectrometría de Masas en Tándem
/
Fitoquímicos
/
Frutas
Tipo de estudio:
Estudio diagnóstico
/
Investigación cualitativa
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2018
Tipo del documento:
Artículo
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