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Clinical value of droplet digital PCR in rapid diagnosis of invasive fungal infection in neonates / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics ; (12): 45-51, 2019.
Artículo en Chino | WPRIM | ID: wpr-776655
ABSTRACT
OBJECTIVE@#To evaluate the clinical value of droplet digital PCR (ddPCR) in rapid and accurate diagnosis of invasive fungal infection (IFI) in neonates.@*METHODS@#The highly conserved sequence of fungi 18S RNA was selected as the target sequence, and primers were designed to establish a ddPCR fungal detection system. Blood samples were collected from 83 neonates with high-risk factors for IFI and/or related clinical symptoms in the neonatal intensive care unit (NICU) of a hospital in Shenzhen, China. Blood culture and ddPCR were used for fungal detection.@*RESULTS@#The ddPCR fungal detection system had a specificity of 100% and a sensitivity of 3.2 copies/μL, and had a good reproducibility. Among the 22 blood samples from neonates with a confirmed or clinical diagnosis of IFI, 19 were detected positive by ddPCR. Among the 61 blood samples from neonates who were suspected of IFI or had no IFI, 2 were detected positive by ddPCR.@*CONCLUSIONS@#The ddPCR technique can be used for the detection of neonatal IFI and is a promising tool for the screening and even diagnosis of neonatal IFI.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: China / Reacción en Cadena de la Polimerasa / Reproducibilidad de los Resultados / Infecciones Fúngicas Invasoras Tipo de estudio: Estudio diagnóstico / Factores de riesgo Límite: Humanos / Recién Nacido País/Región como asunto: Asia Idioma: Chino Revista: Chinese Journal of Contemporary Pediatrics Año: 2019 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: China / Reacción en Cadena de la Polimerasa / Reproducibilidad de los Resultados / Infecciones Fúngicas Invasoras Tipo de estudio: Estudio diagnóstico / Factores de riesgo Límite: Humanos / Recién Nacido País/Región como asunto: Asia Idioma: Chino Revista: Chinese Journal of Contemporary Pediatrics Año: 2019 Tipo del documento: Artículo