Rat Cerebrospinal Fluid Treatment Method through Cisterna Cerebellomedullaris Injection / 神经科学通报·英文版
Neuroscience Bulletin
;
(6): 827-832, 2018.
Artículo
en Inglés
| WPRIM
| ID: wpr-777027
ABSTRACT
Drugs that lack the ability to cross the blood-brain barrier (BBB) need to be placed directly into the central nervous system. Our laboratory studies the involvement of the glutamatergic system in the aggressiveness of glioma, and some ligands of glutamate receptors cannot permeate the BBB. Here, glioma-implanted rats were treated by a technique that delivers ligands directly into the cerebrospinal fluid by puncture into the cisterna cerebellomedullaris. Rats were anesthetized and fixed in a rodent stereotactic device. The head was gently tilted downwards at an angle that allowed exposure of the cisterna. Injection into the cisterna was done freehand using a gingival needle coupled to a microsyringe. The efficiency of intracisternal injection was demonstrated using a methylene blue solution. This type of injection is adaptable for any rodent model using small volumes of a variety of other drugs, and is an interesting method for neuroscience studies.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fármacos del Sistema Nervioso Central
/
Líquido Cefalorraquídeo
/
Cisterna Magna
/
Ratas Wistar
/
Fármacos actuantes sobre Aminoácidos Excitadores
/
Medios de Contraste
/
Modelos Animales
/
Quimioterapia
/
Glioma
/
Anestesia
Límite:
Animales
Idioma:
Inglés
Revista:
Neuroscience Bulletin
Año:
2018
Tipo del documento:
Artículo
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