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Identification of stable reference gene by qRT-PCR in Senecio scandens / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 465-471, 2019.
Artículo en Chino | WPRIM | ID: wpr-777478
ABSTRACT
As a traditional Chinese medicine, Senecio scandens is rich in important compounds such as flavonoid and sesquiterpenoid. Based on the transcriptome data of S. scandens, 15 candidate reference genes were selected including ABCT, ACT1, ACT2, ACT3, ACBP, ARF, ATPS, EF-H, EF-1α, ETIF, GAPDH, GTPB, MPS, UCE and 60S. Firstly, 9 candidate genes with relatively stable expressions such as ACT1, ACBP, ARF, ATPS, EF-1α, GAPDH, MPS, UCE and 60S were screened from different tissues of S. scandens by RT-PCR. Then, qRT-PCR was used to quantitatively analyze gene expression of these nine candidates in S. scandens with or without stress treatments. Further analysis of these gene expression data by geNorm and NormFinder showed that ACT1 exhibited the stablest expression in all samples and could serve as a reference gene for future study of S. scandens, and provide an endogenous control for gene expression analysis.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Plantas Medicinales / Estándares de Referencia / Senecio / Genes de Plantas / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Perfilación de la Expresión Génica / Transcriptoma / Genética / Medicina Tradicional China Tipo de estudio: Estudio diagnóstico Idioma: Chino Revista: China Journal of Chinese Materia Medica Año: 2019 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Plantas Medicinales / Estándares de Referencia / Senecio / Genes de Plantas / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Perfilación de la Expresión Génica / Transcriptoma / Genética / Medicina Tradicional China Tipo de estudio: Estudio diagnóstico Idioma: Chino Revista: China Journal of Chinese Materia Medica Año: 2019 Tipo del documento: Artículo