Role of D-bifunctional protein in promoting the growth of hepatocellular carcinoma in rats and nude mice / 临床肝胆病杂志

ABSTRACT

ObjectiveTo investigate the expression of D-bifunctional protein (DBP) in hepatocellular carcinoma (HCC) tissue in rats and the growth of DBP-induced HCC in nude mice. MethodsA total of 22 male Sprague-Dawley rats were randomly divided into normal control group with 8 rats and model group with 14 rats treated with intraperitoneally injected diethylnitrosamine to induce HCC, and Western blotting, immunohistochemistry, and RT-PCR were used to measure the expression of DBP. A total of 14 specific pathogen-free male BALB/c-nu mice were randomly divided into two groups. HepG2 cells were transfected with empty plasmid or DBP overexpression plasmid and were then injected subcutaneously into nude mice. There were 8 mice in the empty plasmid control group and 6 mice in the DBP high-expression plasmid group, and tumor size was measured for both groups. The t-test was used for comparison of continuous data between groups. ResultsThe rats with HCC had significantly higher protein and mRNA expression of DBP in liver tissue than normal rats (protein: 1.10±0.35 vs 0.67±0.12, t=-7.48, P＜0.05; mRNA: 3.70±0.85 vs 1.17±0.72, t=-20.46, P＜0.05). The DBP high-expression plasmid group had a significantly higher tumor volume than the empty plasmid group ［(7590.50±1867.97)mm3 vs (1663.78±420.24)mm3, t=-39.78, P＜0.01］. ConclusionHighly expressed DBP can promote the progression of HCC in rats and thus provides a new target for the treatment of HCC and the research and development of inhibitory drugs.