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Effect of irradiation on the Porphyromonas gingivalis / 대한구강악안면방사선학회지
Korean Journal of Oral and Maxillofacial Radiology ; : 39-47, 2008.
Artículo en Coreano | WPRIM | ID: wpr-78068
ABSTRACT

PURPOSE:

The aim of this study was to observe a direct effect of irradiation on the periodontopathic Porphyromonas gingivalis (P. gingivalis). MATERIALS AND

METHODS:

P. gingivalis 2561 was exposed to irradiation with a single absorbed dose of 10, 20, 30, and 40 Gy. Changes in viability and antibiotic sensitivity, morphology, transcription, and protein profile of the bacteriumafter irradiation were examined by pour plating method, disc diffusion method, transmission electron microscopy, RT-PCR, and immunoblot, respectively.

RESULTS:

Viability of irradiated P. gingivalis drastically reduced as irradiation dose was increased. Irradiated P. gingivalis was found to have become more sensitive to antibiotics as radiation dose was increased. With observation under the transmission electron microscope, the number of morphologically abnormal cells was increased with increasing of irradiation dose. In RT-PCR, decrease in the expression of fimA and sod was observed in irradiated P. gingivalis. In immunoblot, change of profile in irradiated P. gingivalis was found in a number of proteins including 43-kDa fimbrillin.

CONCLUSION:

These results suggest that irradiation may affect the cell integrity of P. gingivalis, which is manifested by the change in cell morphology and antibiotic sensitivity, affecting viability of the bacterium.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Enfermedades Periodontales / Xerostomía / Proteínas / Porphyromonas / Porphyromonas gingivalis / Microscopía Electrónica de Transmisión / Difusión / Electrones / Antibacterianos Idioma: Coreano Revista: Korean Journal of Oral and Maxillofacial Radiology Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Enfermedades Periodontales / Xerostomía / Proteínas / Porphyromonas / Porphyromonas gingivalis / Microscopía Electrónica de Transmisión / Difusión / Electrones / Antibacterianos Idioma: Coreano Revista: Korean Journal of Oral and Maxillofacial Radiology Año: 2008 Tipo del documento: Artículo