Expression of SDF-1alpha and leptin, and their effect on expression of angiogenic factors in mouse ovaries / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
;
: 135-141, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-78199
ABSTRACT
OBJECTIVE:
Ovarian angiogenesis plays an important role in folliculogenesis. However, little is known about the expression of angiogenic factors during follicular development according to female age. Stromal cell derived factor-1alpha (SDF-1alpha) plays a role in granulosa cell survival and embryo quality as an angiogenic chemokine. Leptin is also involved in folliculogenesis and angiogenesis. This study examined expression of SDF-1alpha and leptin, and their effects on the expression of angiogenic factors in the ovary during follicular development according to female age.METHODS:
Ovaries were collected from C57BL mice of two age groups (6-9 weeks and 24-26 weeks) at 6, 12, 24, and 48 hours after 5 IU pregnant mare's serum gonadotropin (PMSG) injection. The expression of ovarian SDF-1alpha and leptin mRNA was evaluated by RT-PCR. In the organ culture experiment, the ovaries were cultured in transwell permeable supports with Waymouth's medium treated with various doses of SDF-1alpha (50-200 ng/mL) or leptin (0.01-1 microg/mL) for 7 days. Then, mRNA expression of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), and visfatin were examined in the cultured ovaries.RESULTS:
Expression of SDF-1alpha and leptin in the ovary was significantly lower in the aged mouse group compared to the young mouse group (p<0.05). Expression of these two factors increased with follicular development after PMSG administration. SDF-1alpha treatment stimulated visfatin expression in a dose-dependent manner, while leptin treatment significantly increased eNOS expression.CONCLUSION:
These results suggest that decrease of ovarian SDF-1alpha and leptin expression may be associated with aging-related reduction of ovarian function. SDF-1alpha and leptin may play a role in follicular development by regulating the expression of angiogenic factors in mouse ovaries.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Técnicas de Cultivo de Órganos
/
Ovario
/
Envejecimiento
/
ARN Mensajero
/
Células del Estroma
/
Leptina
/
Factor A de Crecimiento Endotelial Vascular
/
Inductores de la Angiogénesis
/
Estructuras Embrionarias
/
Óxido Nítrico Sintasa de Tipo III
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Inglés
Revista:
Clinical and Experimental Reproductive Medicine
Año:
2011
Tipo del documento:
Artículo
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