Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip
Journal of Veterinary Science
;
: 4-2020.
Artículo
en Inglés
| WPRIM
| ID: wpr-782521
ABSTRACT
detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infected bees (Apis mellifera), and then, within 10 min, amplified the target cDNA by ultra-rapid qPCR. The PCR products were hybridized to a DNA-chip for confirmation of target gene specificity. The results of this study suggest that our method might be a useful tool for detecting DWV, as well as for the diagnosis of RNA virus-mediated diseases on-site.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Abejas
/
ARN
/
ARN Viral
/
Reacción en Cadena de la Polimerasa
/
Sensibilidad y Especificidad
/
ADN Complementario
/
Diagnóstico
/
Apicultura
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2020
Tipo del documento:
Artículo
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