Effect of Iron-chelator on the Neurotoxicity Induced by Oxygen Radicals / 체질인류학회지

ABSTRACT

In order to elucidate the neurotoxicity of oxygen radicals, neurotoxic effect was investigated after cultured mouse spinal sensory ganglionic cells were exposed to oxygen radicals which were generated enzymatically by reaction of xanthine oxidase (XO) and hypoxanthine (HX) in culture medium. And also the neuroprotective effect of iron-chelators against oxidant-induced neurotoxicity was assessed by MTT assay and neurofilament enzymeimmuno assay (EIA). Cell viability was significantly decreased in a time-dependent planner after exposure of cultured neurons to 25mU/ml XO and 0.3mM HX for 3 hours. In the examination of neuroprotective effect of iron-chelators on oxidant-mediated neurotoxicity. TPEN was effective in blocking the neurotoxicity induced by oxygen radicals, while DFX did not showed any neuroprotective effect in these cultures. These results suggest that oxygen radicals are toxic in cultured mouse spinal sensory ganglionic cells, and also iron involves in oxidant-induced neurotoxicity. While, selective iron-chelators such as TPEN are effective in blocking the neurotoxicty induced by oxygen radicals.