The Effect of Bioplant HTR(R)(Hard Tissue Replacement) on Osteogenesis of The Mandibular Bone Defects in Rabbits

ABSTRACT

Tissue Replacement) on osteogenesis in the mandibular bone defects. Eight adult male white rabbits weighing 2.5 to 3.0kg were used. Four bone defects (8mm in diameter and 4mm in depth) were made at the both mandibular body. In the control group, the right mesial bone defect was filled with blood clot and spontaneously healed. In the DFDB group, the right distal bone defect was filled with xenogenic demineralized freeze-dried bone. In the HTR group, the left mesial bone defect was filled with HTR. In the HTR-membrane group, the left distal bone defect was filled with HTR and covered with BioMesh membrane. The rabbits were sacrified at 2, 4, 6 and 9 weeks after the operation and microscopic examination was performed. Results obtained were as follows In the control and DFDB groups, inflammatory cells and the fibrous connective tissue existed and the bone growth was slower than HTR group by 6 week, and there was intervention of the soft tissue at 9 week. In the HTR group, bone trabeculi extended between the HTR particles without intervention of inflammatory cells and the connective tissue at 4 and 6 weeks. In addition, extensive osseous ingrowth into the HTR particles was observed at 9 week. Bone formation was more active in the HTR group than the control and DFDB groups. There was not obvious difference in the bone healing rate between the HTR and the HTR-membrane group. These results suggest that the HTR promotes osteogenesis in the bone defects and the HTR group has no difference in comparison with the HTR-BioMesh membrane group in bone healing.