A STUDY OF TGF-Beta EXPRESSION DURING PALATOGENESIS IN RATS WITH CLEFT PALATE INDUCED BY BAPN

ABSTRACT

Cleft palate is one of the most serious congenital anomalies in human that causes a sucking problem in newborn babies and phologic deformity that usually leads to death in newborn mouse offspring due to an insufficient ability to suck milk. Therefore cleft palate had been researched with epidemiologic and molecular methods, and many etiologic factors were examined closely. Among of the research methods, biologic molecule researches have been more important method for cleft palate formation study. The TGF-Beta had an important role in the cell migration, epithelial- mesenchymal transdifferentiation, extracellular matrix synthesis and deposition. But there was a little research which was study about correlation cleft palate induced by beta-aminonitroproprionitrile (BAPN) with TGF-Beta expression. A purpose of this presented study was examed how TGF-Beta expression in cleft palate mice. At gestation days 13, BAPN-monofumarate salts ((C3H6N2)2 C4H4O4, Sigma Co.) was single oral administered to 4 pregnant rats according to 1g/kg body weight. And pregnant rats were sacrificed on day 20 post coitus (p.c.), The TGF-Beta expression patterns of cleft formed fetus mice was followed that; 1. Osteoblast, mesenchymal cell and epithelial cell of cleft mice were low expression compare to control mice. 2. There was no TGF-Beta difference expression pattern of osteocyte of cleft mice compare to control mice. 3. In western blot analysis, thickness of band of TGF-Beta in cleft mice was thin and dilute compare to control mice.