THE DIFFERENCE IN THE PREVALENCE OF THE IVS5-A/G OF THE TRANSFORMING GROWTH FACTOR-BETA3 GENE BETWEEN KOREANS AND ROMANIANS

ABSTRACT

prevalence of the IVS5-A/G of the TGF-beta3 gene in the Korea and the Romania. The TGF-beta3 genotypes of 151 randomly selected common populations were analyzed using PCR amplification, SNaf1 restriction enzyme digestion, electrophoresis, and direct sequencing. The frequency of the substitution was calculated in different genders and statistically analyzed (Chisquare). Among 87 in Koreans (male 49, female 38), about 47% had an A/A homozygous genotype, 41% had a A/G heterozygous genotype, and the remaining 12% had a G/G homozygous genotype. The frequencies for the A and G alleles were 66% (p) and 34% (q), respectively. Among 64 in Romanians (male 27, female 37), about 78% had an A/A homozygous genotype, 20% had a A/G heterozygous genotype, and the remaining 2% had a G/G homozygous genotype. The frequencies for the A and G alleles were 87% (p) and 13% (q), respectively. There were significant differences between Koreans and Romanians (P<0.001). The study provides a fair estimate for the prevalence of the IVS5-A/G substitution of the TGF-beta3 gene in the general population in the Korea and the Romania, which will facilitate further investigations of the pathogenic effects of the gene.