Silk Fibroin and Substance P Combination Graft for the Reconstruction of a Bone Defect
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
;
: 293-300, 2011.
Artículo
en Coreano
| WPRIM
| ID: wpr-785089
ABSTRACT
PURPOSE: Substance P is a well known neurotransmitter and has been known to mediate pain. Recently, it has been unveiled that substance P is involved in the recruitment of mesenchymal stem cells to wound sites. The purpose of this study was to exam bone formation when a combination of substance P and silk fibroin was used in a bone defect model.METHODS:
Twenty rabbits were used and 40 calvarial defects were formed. They were divided as 4 groups (unfilled control, silk only, silk+10microg/ml substance P; Sub10, and silk+100microg/ml substance P; Sub100). All animals were humanely sacrificed 4 or 8 weeks after grafting. The specimens were analyzed by micro-computerized tomography and histological analysis.RESULTS:
When compared to the unfilled control to silk only group, there was significant difference in bone mineral density (BMD) and the attenuation coefficient (AC) at 4 weeks (P=0.037 and 0.038, respectively). When compared Sub10 group to Sub100 group, there was significant difference in BMD and AC at 8 weeks (P=0.004 for all). Residual graft amounts were 52.1+/-15.8%, 15.2+/-9.2% and 9.0+/-3.3% for silk only, Sub10, and Sub100 groups, respectively. When comparing the residual graft amount of silk only to sub10 or sub100, the differences were statistically significant (P<0.001).CONCLUSION:
The silk fibroin scaffold showed higher BMD and AC than the unfilled control. The combination graft with substance P and silk fibroin scaffold showed a faster graft degradation than with a silk fibroin scaffold only.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteogénesis
/
Sustancia P
/
Densidad Ósea
/
Neurotransmisores
/
Trasplantes
/
Seda
/
Células Madre Mesenquimatosas
/
Fibroínas
Límite:
Animales
/
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
Año:
2011
Tipo del documento:
Artículo
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