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In vivo Osteogenesis of Cultured Human Periosteal-derived Cells and Polydioxanone/Pluronic F127 Scaffold
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons ; : 384-390, 2012.
Artículo en Coreano | WPRIM | ID: wpr-785191
ABSTRACT
PURPOSE: The purpose of this study is to examine in vivo osteogenesis of cultured human periosteal-derived cells and polydioxanone/pluronic F127 scaffold.

METHODS:

Two one-year-old miniature pigs were used in this study. 2x106 periosteal-derived cells in 1 mL medium were seeded by dropping the cell suspension into the polydioxanone/pluronic F127 scaffold. These cell-scaffold constructs were cultured in osteogenic Dulbecco's modified Eagle's medium for 7 days. Under general anesthesia with azaperone and tiletamine-zolazepam, the mandibular body and ramus of the pigs were exposed. Three bony defects were created. Polydioxanone/pluronic F127 scaffold with periosteal-derived cells and the scaffold only were implanted into each defect. Another defect was left empty. Twelve weeks after implantation, the animals were sacrificed.

RESULTS:

New bone formation was clearly observed in the polydioxanone/pluronic F127 scaffold with periosteal-derived cells. Newly generated bone was also observed in the scaffold without periosteal-derived osteoblasts and empty defect, but was mostly limited to the periphery.

CONCLUSION:

These results suggest that cultured human periosteal-derived cells have good osteogenic capacity in a polydioxanone/pluronic F127 scaffold, which provides a proper environment for the osteoblastic differentiation of these cells.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteoblastos / Osteogénesis / Polietilenos / Polipropilenos / Semillas / Azaperona / Porcinos / Durapatita / Anestesia General Límite: Animales / Humanos Idioma: Coreano Revista: Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons Año: 2012 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteoblastos / Osteogénesis / Polietilenos / Polipropilenos / Semillas / Azaperona / Porcinos / Durapatita / Anestesia General Límite: Animales / Humanos Idioma: Coreano Revista: Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons Año: 2012 Tipo del documento: Artículo