Exploratory, cognitive, and depressive-like behaviors in adult and pediatric mice exposed to controlled cortical impact
Clinical and Experimental Emergency Medicine
;
(4): 125-137, 2019.
Artículo
en Inglés
| WPRIM
| ID: wpr-785606
ABSTRACT
OBJECTIVE:
Sequelae of behavioral impairments associated with human traumatic brain injury (TBI) include neurobehavioral problems. We compared exploratory, cognitive, and depressive-like behaviors in pediatric and adult male mice exposed to controlled cortical impact (CCI).METHODS:
Pediatric (21 to 25 days old) and adult (8 to 12 weeks old) male C57Bl/6 mice underwent CCI at a 2-mm depth of deflection. Hematoxylin and eosin staining was performed 3 to 7 days after recovery from CCI, and injury volume was analyzed using ImageJ. Neurobehavioral characterization after CCI was performed using the Barnes maze test (BMT), passive avoidance test, open-field test, light/dark test, tail suspension test, and rotarod test. Acutely and subacutely (3 and 7 days after CCI, respectively), CCI mice showed graded injury compared to sham mice for all analyzed deflection depths.RESULTS:
Time-dependent differences in injury volume were noted between 3 and 7 days following 2-mm TBI in adult mice. In the BMT, 2-mm TBI adults showed spatial memory deficits compared to sham adults (P < 0.05). However, no difference in spatial learning and memory was found between sham and 2-mm CCI groups among pediatric mice. The open-field test, light/dark test, and tail suspension test did not reveal differences in anxiety-like behaviors in both age groups.CONCLUSION:
Our findings revealed a graded injury response in both age groups. The BMT was an efficient cognitive test for assessing spatial/non-spatial learning following CCI in adult mice; however, spatial learning impairments in pediatric mice could not be assessed.
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Lesiones Encefálicas
/
Eosina Amarillenta-(YS)
/
Suspensión Trasera
/
Prueba de Desempeño de Rotación con Aceleración Constante
/
Aprendizaje Espacial
/
Memoria Espacial
/
Hematoxilina
/
Aprendizaje
/
Memoria
Límite:
Animales
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Clinical and Experimental Emergency Medicine
Año:
2019
Tipo del documento:
Artículo
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