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Comparison of Gene Expression from Supernumerary Dental Pulp and Periodontal Ligament Stem Cells / 대한소아치과학회지
Journal of Korean Academy of Pediatric Dentistry ; (4): 242-249, 2018.
Artículo en Coreano | WPRIM | ID: wpr-787305
ABSTRACT
The purpose of this study is to compare the properties of dental pulp and periodontal ligament stem cells from extracted supernumerary teeth by quantitative real-time PCR.Impacted supernumerary teeth in the maxillary anterior region were extracted. Dental pulp and periodontal ligament cells were collected from extracted supernumerary teeth on the same day. After isolation and culture of cells, compare characterization of them by using qRT-PCR. Primer sequences for odontoblasts are ONT, ALP, OCN, DMP-1 and DSPP.On dental pulp group, ONT has the largest quantity of gene expression, followed by OCN, ALP, DMP-1 and DSPP. On periodontal ligament group, ONT has the largest quantity of gene expression, followed by OCN, ALP, DSPP and DMP-1. Analysis of quantitative gene expression data using relative quantification showed that the expression of all genes decreased in periodontal ligament cells.Dental pulp and periodontal ligament stem cells from supernumerary teeth have the properties of odontoblasts. Considering that properties, supernumerary teeth were considered a useful donor site of dental pulp and periodontal ligament stem cells.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ligamento Periodontal / Células Madre / Donantes de Tejidos / Diente Supernumerario / Expresión Génica / Pulpa Dental / Reacción en Cadena en Tiempo Real de la Polimerasa / Odontoblastos Límite: Humanos Idioma: Coreano Revista: Journal of Korean Academy of Pediatric Dentistry Año: 2018 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ligamento Periodontal / Células Madre / Donantes de Tejidos / Diente Supernumerario / Expresión Génica / Pulpa Dental / Reacción en Cadena en Tiempo Real de la Polimerasa / Odontoblastos Límite: Humanos Idioma: Coreano Revista: Journal of Korean Academy of Pediatric Dentistry Año: 2018 Tipo del documento: Artículo