Partition bands counting established for PFEG fingerprint analysis / 上海预防医学

ABSTRACT

To establish a new method for PFGE fingerprint analysis , which is easy-to-operate , inexpensive , software-low-dependent and readily-exchangeable . [ Methods ] A group of 44 vibrio parahaemolyticus was subtyped according to PulseNet standardized PFGE protocol .The fingerprint was analyzed with new gel-partition bands counting method and BioNumerics clustering separately and then the results by the two methods were compared with type number , cluster number and Simpson ’ s Index of Diversity ( DI) . [ Results] The 44 isolates could be typed into 32 types and 5 clusters were found by gel-partition bands counting method ( DI=97.6%) .BioNumerics could type the same isolates into 29 types and 4 clusters were found ( DI=95.5%) .The difference in the ability of finding clusters between the two methods was not statistically significant . [ Conclusion] Gel-partition bands counting method is based on the bands distribution among finger print and greatly reduces the number of visually observed spectrum , which is easy-to-operate, inexpensive , software-low-dependent and readily-exchangeable .