A study on the effect of indomethacin on SiO2 induced lysozyme over-expression in rat alveolar macrophages / 预防医学

ABSTRACT

Objective To learn the effect of indomethacin (IND)of different concentrations on the expression of lysozyme protein induced by SiO2 in rat alveolar macrophages (AM).Methods Pure AM was prepared with the method of bronchoalveolar lavage in rats.In the model group,the silica dust poisoning model was replicated by adding SiO2 dust suspension (50 μg /ml).In the intervention group,following the adding of SiO2 dust suspension,the IND of 1 0 -5 ,1 0 -4 , and 1 0 -3 g/ml were added respectively.In the control group,the same volume of PBS was given.After 8 h,1 6 h,the cell morphology was observed.Results Compared with the model group,the AM cells in the intervention group were relatively complete,and that there was a concentration dependent trend.The expression of LSZ protein in AMof the model group was significantly higher than that of the control group (P<0. 05 ),while the expression of LSZ protein in the intervention group decreased compared with that of the model group.After incubation with IND and SiO2 ,the expression of LSZ protein in the intervention group decreased compared with that of the model group.Conclusion IND can inhibit the increased expression of LSZ protein in AMcaused by the stimulation of SiO2dust,and can reduce the damage of SiO2on the membrane of alveolar macrophage and thus has the protective effect on the AM cell membrane.