Effects of propofol and etomidate on hydrogen peroxide-induced oxidative damage in hepatocyte / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 331-336, 2009.
Artículo
en Coreano
| WPRIM
| ID: wpr-79310
ABSTRACT
BACKGROUND:
The present investigation was undertaken to evaluate the protective effect of propofol and etomidate against hydrogen peroxide (H2O2) induced oxidative damage in human hepatic SNU761 cells by measuring lactate dehydrogenase (LDH).METHODS:
The cell line of human hepatocellular carcinoma was grown for 24 hours in dissociated cell culture. They were divided into eight groups negative control (NC) group with no drug administration, positive control (PC) group with H2O2 250 micrometer and other groups pretreated with propofol (P; 1, 10, 50 micrometer) or etomidate (ET; 1, 10, 50 micrometer) followed H2O2 administration. After 7 hours, cell death was assessed by morphology under the light microscope and quantified by measuring the LDH in the culture media.RESULTS:
In the light microscopic findings, the intact cells were increased in all three propofol groups compared to group PC. H2O2-induced LDH production was also significantly suppressed in all three propofol groups compared to group PC (P < 0.001). There were no significant differences in the microscopic findings and LDH production between the etomidate groups and group PC.CONCLUSIONS:
These results suggest that the propofol has protective effect on the hepatocyte against H2O2-induced oxidative stress.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Propofol
/
Línea Celular
/
Muerte Celular
/
Carcinoma Hepatocelular
/
Estrés Oxidativo
/
Técnicas de Cultivo de Célula
/
Medios de Cultivo
/
Hepatocitos
/
Etomidato
/
Hidrógeno
Límite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Anesthesiology
Año:
2009
Tipo del documento:
Artículo
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