Enhancement of O-dealkylation in Mouse Liver by Dietary Administrations of BHA and BHT: Studies with Isolated Perfused Livers and Hepatic Microsomes
Yonsei Medical Journal
;
: 106-113, 1986.
Artículo
en Inglés
| WPRIM
| ID: wpr-79331
ABSTRACT
Effects of feeding 2(3)-tert-butyl 4-hydroxyanisole (BHA) and 3, 5-di-tert-butyl 4-hydroxytoluene (BHT) on the rates of mixed function oxidation and conjugation enzyme reactions have been determined using isolated hepatic microsomal fractions and isolated perfused livers of mice. The treatments with either of the antioxidants have increased the rates of O-demethylation for p-nitroanisole and of O-deethylation for 7-ethoxycoumarin up to 2-fold, both in microsomes and in perfused liver. Analysis of the perfusate showed that the increased amounts of p-nitrophenol and 7-hydroxycoumarin produced by the elevated mixed-function oxidase activities were reflected by the increase in the amounts of glucuronide conjugates and not in the increase for the amounts of the sulfate ester conjugates. Comparison of results also indicated that in the perfused liver, the maximal rate of metabolite conjugation is limited by the maximal rates of the initial mixed function oxidase activities.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxidación-Reducción
/
Perfusión
/
Hidroxianisol Butilado
/
Hidroxitolueno Butilado
/
Microsomas Hepáticos
/
Estudio Comparativo
/
Glucuronosiltransferasa
/
Cumarinas
/
Alquilación
/
Oxigenasas de Función Mixta
Límite:
Animales
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
1986
Tipo del documento:
Artículo
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