Antigenic and immunogenic investigation of the virulence motif of the Newcastle disease virus fusion protein
Journal of Veterinary Science
;
: 205-211, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-79618
ABSTRACT
Newcastle disease (ND) caused by virulent Newcastle disease virus (NDV) is a highly contagious viral disease of poultry. Virulent NDVs characteristically have a multibasic amino acid sequence (virulence motif) such as (112)RRQKRF(117) at the cleavage site of the precusor fusion (F0) protein. The antigenic and immunogenic characteristics of the virulence motif (112)RRQKRF(117) in the F0 protein of virulent NDVs were investigated. Epitope mapping analysis revealed that a RRQKRF-specific monoclonal antibody 4G2 recognized the KRF section of the motif. A synthetic peptide bearing the RRQKRF motif reacted strongly with sera from virulent NDV (with RRQKRF motif)-infected chickens. These sera also showed reactivity to peptides bearing other virulence motifs ((112)KRQKRF(117), (112)RRQRRF(117) and (112)RRRKRF(117)) but not an avirulence motif ((112)GRQGRL(117)) by ELISA. The synthetic bearing RRQKRF motif reacted with 60% to 91% of sera taken from surviving chickens on ND outbreak farms but not with sera from vaccinated birds, even though most of the sera had antibody to NDV due to vaccination. This indicates that the virulence motif has the potential to differentiate virulent NDV infected birds from vaccinated birds.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Enfermedades de las Aves de Corral
/
Virulencia
/
Virus de la Enfermedad de Newcastle
/
Ensayo de Inmunoadsorción Enzimática
/
Pruebas Serológicas
/
Pollos
/
Proteínas Virales de Fusión
/
Secuencia de Aminoácidos
/
Mapeo Epitopo
/
Secuencias de Aminoácidos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2010
Tipo del documento:
Artículo
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