The Kinetics of Secondary Response of Antigen-Specific CD4+ T Cells Primed in vitro with Antigen
Immune Network
;
: 93-101, 2006.
Artículo
en Coreano
| WPRIM
| ID: wpr-79619
ABSTRACT
BACKGROUND:
Memory T lymphocytes of the immune system provide long-term protection in response to bacterial or viral infections/immunization. Ag concentration has also been postulated to be important in determining whether T cell differentiation favors effector versus memory cell development. In the present study we hypothesized that na?ve Ag-specific CD4+ T cells briefly stimulated with different Ag doses at the primary exposure could affect establishment of memory cell pool after secondary immunization.METHODS:
To assess this hypothesis, the response kinetics of DO11.10 TCR CD4+ T cells primed with different Ag doses in vitro was measured after adoptive transfer to naive BALB/c mice.RESULTS:
Maximum expansion was shown in cells primarily stimulated with high doses of ovalbumin peptide (OVA323-339), whereas cells in vitro stimulated with low dose were expanded slightly after in vivo secondary exposure. However, the cells primed with low OVA323-339 peptide dose showed least contraction and established higher number of memory cells than other treated groups. When the cell division was analyzed after adoptive transfer, the high dose Ag-stimulated donor cells have undergone seven rounds of cell division at 3 days post-adoptive transfer. However, there was very few division in naive and low dose of peptide-treated group.CONCLUSION:
These results suggest that primary stimulation with a low dose of Ag leads to better memory CD4+ T cell generation after secondary immunization. Therefore, these facts imply that optimally primed CD4+ T cells is necessary to support effective memory pool following administration of booster dose in prime-boost vaccination.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Donantes de Tejidos
/
Cinética
/
Linfocitos T
/
Diferenciación Celular
/
División Celular
/
Ovalbúmina
/
Inmunización Secundaria
/
Vacunación
/
Traslado Adoptivo
/
Sistema Inmunológico
Límite:
Animales
/
Humanos
Idioma:
Coreano
Revista:
Immune Network
Año:
2006
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS