Effect of taurine on the antioxidant capacity and ATP activity of skeletal muscle in rats with exhaustive exercise / 中国基层医药

ABSTRACT

Objective@#To explore the effect of taurine on the activity of SOD, MDA, Na+ -K+ -ATP enzyme and Bax/Bcl-2 in skeletal muscle of rats after exhaustive exercise.@*Methods@#Thirty male SD rats(6 months old) were randomly divided into normal group, control group and taurine group, with 10 rats in each group.The normal group was given routine feeding for 1 week, adaptive training for 3 days, without applying other measures.The control group was given routine feeding for 1 week, after adaptive training for 3 days underwent exhaustive exercise for 2 days.The taurine group was given 200mg/kg taurine gavage daily on the basis of the control group.The activity of SOD, MDA, Na+ -K+ -ATP and Bax/Bcl-2 were measured in the skeletal muscle after 2 days exhaustive exercise.@*Results@#After 2 days exhaustive exercise, the SOD concentration in skeletal muscle of the control group was (146.58±13.42)U/mg prot, which was lower than that in the taurine group[(143.81±15.93)U/mg prot] (t=2.519, P<0.05). The MDA concentration in the skeletal muscle of the control group was (1.97±0.20)nmol/mg prot, which was higher than that in the taurine group [(1.22±0.19)nmol/mg prot] (t=2.356, P<0.05). The ratio of SOD/MDA in the control group was (60.86±20.38), which was lower than that in the taurine group [(120.87±23.51)] (t=4.071, P<0.05). The activity of Na+ -K+ -ATP in the control group was (2.42±0.67)U/mg prot, which was lower than that in the taurine group [(5.74±1.15)U/mg prot] (t=3.905, P<0.05). The ratio of Bax/Bcl-2 in the control group was (1.62±0.17), which was higher than that in the taurine group [(0.96±0.14)] (t=3.419, P<0.05).@*Conclusion@#Taking taurine before exhaustive exercise can increase the ability of scavenging free radicals, reduce the production of oxidative stress products and protect the activity of Na+ -K+ -ATP enzyme.It is benefit for maintain the stability of cell environment and prevent skeletal muscle injury.