Analysis the methylation of caveolin-2 gene in peripheral blood of uygur pigeon feeders / 中国医师进修杂志

ABSTRACT

Objective@#To study the methylation status of caveolin-2(CAV2) gene in peripheral blood of uygur pigeon breeder lung patients, and discuss the significance of methylation of CAV2.@*Methods@#Twenty cases of uygur people who suffered from diseases after raising pigeons were enrolled into case group; 20 Urgur pigeons without disease were enrolled into case control group; and 20 uygur healthy subjects without pigeon were enrolled into healthy control group. General data of 60 subjects were collected and peripheral blood samples were collected. DNA was extracted from the retained peripheral blood samples, followed by hydrogen sulfite transformation, PCR amplification, in vitro transcription and RNase A-specific enzyme digestion, and finally, matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to detect the methylation of CAV2.@*Results@#CpG site of CAV2 fragment(CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_9, CpG_10, CpG_11), actually detected 9 sites (CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_11), the methylation rate distribution of each site in the three groups showed no statistical differences (P > 0.05). The methylation rates of each site between the three groups were compared in pairs, and showed no statistical differences (P > 0.05).@*Conclusions@#Whether CAV2 gene methylation has any effect on the pulmonary pathogenesis and pulmonary fibrosis process of uygur pigeon feeders remains to be further studied.