Angiogenesis Activity of Morindae Officinalis Radix from Different Origins Based on Zebrafish Model / 中国实验方剂学杂志

ABSTRACT

Objective: To study the effect of Morindae Officinalis Radixe extract from different origins on the vascular growth of damaged internodes of zebra fish, so as to discuss regional differences of Morindae Officinalis Radix from different origins. Method: A zebra fish model of internode vascular injury induced by PTK787 was established to study the effect of Morindae Officinalis Radix extract from Guangdong, Guangxi and Fujian on the growth of internode vascular in zebra fish, and detect the number of internode angiogenesis in zebra fish treated with PTK787.On the basis of principal component analysis, relative angiogenesis rate was taken as the index, and the experimental data was analyzed by SPSS 17.0 statistical software. Result: The extract of Morindae Officinalis Radix had a repairing effect on the growth of damaged internode blood vessels of zebra fish, and the extracts of Morindae Officinalis Radix from Guangdong Gaoyao, and Guangdong Yunan, were better than those from other producing areas. The average number of internode angiogenesis was 9, the average length of angiogenesis was 448-504 μm, and the relative rate of angiogenesis was between 7.9% and 8.86%. The results of cluster analysis showed that Morindae Officinalis Radix of 12 different origins were classified into four categories, Guangdong Deqing, and Guangdong Gaoyao, Guangxi Cangwu and Guangxi Cenxi, and Guangdong Yunan, and Guangxi Cangwu could be better clustered. Conclusion: There are obvious regional differences in the pro-angiogenic effect of Morindae Officinalis Radix from different origins. Relevant studies provide basic data for the quality assessment of Morindae Officinalis Radix medicinal materials.