Suppression of IL-1beta-induced MUC5AC Gene Expression by Ginkgo biloba Extract(EGb 761) in Human Airway Epithelial Cells
Journal of Rhinology
;
: 49-55, 2007.
Artículo
en Coreano
| WPRIM
| ID: wpr-80475
ABSTRACT
BACKGROUND AND OBJECTIVES:
The purpose of this study is to investigate whether Ginkgo biloba extract (EGb 761) can suppress IL-1beta-induced MUC5AC gene expression in NCI-H292 human airway epithelial cells and to discover what its possible mechanism is. MATERIALS ANDMETHODS:
NCI-H292 human pulmonary mucoepidermoid carcinoma cell line was used. MUC5AC mRNA and protein were measured using the RT-PCR and Western blot analysis. The activation of mitogen activated protein kinases (MAPKs) were determined by means of the Western blot analysis.RESULTS:
MUC5AC was induced by treating the NCI-H292 cells with 10 ng/ml of IL-1beta for 24 hours. A pre-treatment of 200 microgram/ml of EGb 761 significantly suppressed the IL-1beta induced MUC5AC expression. The inhibition of MUC5AC gene expression by EGb 761 was noted to be suppressed via both the extracellular signal-regulated kinase (ERK) and the p38 MAPK pathways in a kinase-specific inhibitor study.CONCLUSION:
EGb 761 suppresses IL-1beta-induced MUC5AC gene expression in the human airway epithelial cells. Therefore, it may be considered as a possible anti-hypersecretory agent.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosfotransferasas
/
ARN Mensajero
/
Expresión Génica
/
Línea Celular
/
Western Blotting
/
Carcinoma Mucoepidermoide
/
Ginkgo biloba
/
Proteínas Quinasas Activadas por Mitógenos
/
Proteínas Quinasas p38 Activadas por Mitógenos
/
Células Epiteliales
Límite:
Humanos
Idioma:
Coreano
Revista:
Journal of Rhinology
Año:
2007
Tipo del documento:
Artículo
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