Morphological changes of liver in obese rats induced by high fat diet and its significance / 中华内分泌外科杂志

ABSTRACT

Objective@#To observe the liver histology change of obesity rat induced by high fat diet and discuss its significance.@*Methods@#The rats were divided into 2 groups12 obesity rats induced by high fat diet and 8 rats for control. Then the fasting blood glucose, lipid, blood uric acid, insulin level were tested while BMI, HOMA-IR and ISI were calculated at 14 week. Also the HE staining of the liver tissue was performed.@*Results@#The baseline parameters including gender, body weigh, body length, tail length and Lee’s Index were similar between the two groups (P>0.05) . But after 14 weeks, the body weigh, body length and Lee’s Index of the obesity rats were higher significantly than the normals[ (367.92±88.88) g vs (252.5±59.47) g, (23.14±2.18) cm vs (20.81±1.71) cm, (328.18±9.82) vs (265.00±38.17) , (P<0.05) ]. The fasting blood glucose of obesity group was higher than that of control group, but the HDL level of obesity group was lower than that of control group[ (7.00±1.81) mmol/L vs (4.21±1.13) mmol/L, (1.24±0.14) mmol/L vs (1.41±0.15) mmol/L, (P<0.05) ]. The insulin, triglycerides, cholesterol, LDL, urimic acide, HOMA-IR and ISI were not different between the two groups. The balloon degeneration and fatty degeneration of the liver tissue were observed under light microscope at the obesity group.@*Conclusion@#The balloon-like changes and steatosis of the liver tissue, as well as the high blood sugar and high-density lipoprotein, were the basis of obesity induced abnormal metabolism including fatty liver and glycolipids.