Analysis of human papillomavirus infection and p53 expression in patients with hypopharynx squamous cell carcinoma / 中华实验和临床病毒学杂志

ABSTRACT

Objective@#To analyze the infection of human papillomavirus and expression of p53 in patients with hypopharynx squamous cell carcinoma.@*Methods@#The pathological samples of 15 clinically diagnosed hypopharynx cancer patients were collected. Lunimex and PCR techniques were used to detect the human papillomavirus (HPV) gene and immunohistochemical method was used to analyze the HPV protein expression in the samples. PCR and direct sequencing were used to screen the mutations in p53 gene and immunohistochemistry was used to evaluate the expressing profiles of p53 proteins.@*Results@#In the 15 cases, 6 were positive for HPV16/18 gene and 2 cases were positive for HPV16/18 E6 protein expression. The total positive rate was about 46.7% (7/15). In addition, 9 of 15 (60.0%) cases had sequencing-confirmed p53 mutations and p53 immunohistochemistry (IHC) confirmed 9 (60.0%) cases had positive staining.@*Conclusions@#The high HPV infection rate and p53 expression in hypopharynx squamous cell carcinoma patients in the study lays a foundation for further elucidating the pathogenesis of this kind of cancer.