The effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts / 中华整形外科杂志

ABSTRACT

Objective@#To explore the effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts and clarify the possible role of β5-integrin(ITGB5) in keloid.@*Methods@#Construct lentiviral sh-RNA-expression vector targeting ITGB5 and infect keloid fibroblasts, the expression of ITGB5 were detected by Western Blot, the proliferation ability was identified by MTT.@*Results@#The expression quantity of ITGB5 mRNA and protein in KFb group, LV-NC group and LV-KFb group are 1.00±0.00, 1.08±0.05, 0.34±0.01 and 0.91±0.03, 0.93±0.02, 0.28±0.07. Compared with LV-NC group and KFb group, the expression quantity of ITGB5 mRNA and protein in LV-KFb group decreased significantly(P<0.01). Compared with LV-NC group and KFb group, the proliferation rate decreased significantly in LV-KFb group at 48 h(P<0.01).@*Conclusions@#These results suggest that ITGB5 can accelerate fibroblasts proliferation in keloids.