Comparison of the AdvanSure Human Papillomavirus Screening Real-Time PCR, the Abbott RealTime High Risk Human Papillomavirus Test, and the Hybrid Capture Human Papillomavirus DNA Test for the Detection of Human Papillomavirus
Annals of Laboratory Medicine
;
: 201-205, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-80823
ABSTRACT
BACKGROUND:
We evaluated the performance of various commercial assays for the molecular detection of human papillomavirus (HPV); the recently developed AdvanSure HPV Screening real-time PCR assay (AdvanSure PCR) and the Abbott RealTime High Risk HPV PCR assay (Abbott PCR) were compared with the Hybrid Capture 2 HPV DNA Test (HC2).METHODS:
All 3 tests were performed on 177 samples, and any sample that showed a discrepancy in any of the 3 tests was genotyped using INNO-LiPA HPV genotyping and/or sequencing. On the basis of these results, we obtained a consensus HPV result, and the performance of each test was evaluated. We also evaluated high-risk HPV 16/18 detection by using the 2 real-time PCR assays.RESULTS:
Among the 177 samples, 65 were negative and 75 were positive in all 3 assays; however, the results of the 3 assays with 37 samples were discrepant. Compared with the consensus HPV result, the sensitivities and specificities of HC2, AdvanSure PCR, and Abbott PCR were 97.6%, 91.7%, and 86.9% and 83.9%, 98.8%, and 100.0%, respectively. For HPV type 16/18 detection, the concordance rate between the AdvanSure PCR and Abbott PCR assays was 98.3%; however, 3 samples were discrepant (positive in AdvanSure PCR and negative in Abbott PCR) and were confirmed as HPV type 16 by INNO-LiPA genotyping and/or sequencing.CONCLUSIONS:
For HPV detection, the AdvanSure HPV Screening real-time PCR assay and the Abbott PCR assay are less sensitive but more specific than the HC2 assay, but can simultaneously differentiate type 16/18 HPV from other types.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Papillomaviridae
/
Juego de Reactivos para Diagnóstico
/
ADN Viral
/
Cuello del Útero
/
Sensibilidad y Especificidad
/
Infecciones por Papillomavirus
/
Papillomavirus Humano 16
/
Papillomavirus Humano 18
/
Genotipo
Tipo de estudio:
Estudio diagnóstico
/
Estudio de etiología
/
Estudio de tamizaje
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
Idioma:
Inglés
Revista:
Annals of Laboratory Medicine
Año:
2012
Tipo del documento:
Artículo
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