Establishment of a real-time PCR method to identify Ekpoma virus gene in blood sample of a returnee from Angola / 中华实验和临床病毒学杂志

ABSTRACT

Objective@#To establish quantitative real-time PCR (qPCR) method based on Taqman probe for detecting Ekpoma virus (EKV).@*Methods@#According to the conserved region of gene in EKV genome from GenBank, primers and probe for qPCR were designed. Validity and sensitivity were evaluated in this study. Both whole blood and serum of a returnee from Angola were tested by the established EKV-1 and EKV-2 qPCR method .@*Results@#Sensitivity of EKV-1 and EKV-2 qPCR method was respectively 41 copies/μl and 70 copies/μl. Coefficient of variance (CV) was respectively 1.27%, 0.20%, 0.82%; 2.12%, 1.74%, and 1.40%. EKV-2 gene was detected in both whole blood and serum of a returnee from Angola.@*Conclusions@#The first EKV-2 gene was confirmed in both whole blood and serum of a returnee from Angola by real-time RT-PCR..