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Optimization of critical medium components for enhancing antibacterial thiopeptide nocathiacin I production with significantly improved quality / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.) ; (6): 292-300, 2017.
Artículo en Inglés | WPRIM | ID: wpr-812112
ABSTRACT
Nocathiacin I, a glycosylated thiopeptide antibiotic, displays excellent antibacterial activities against multidrug resistant bacterial pathogens. Previously, a novel nocathiacin I formulation for intravenous administration has been successfully developed and its aqueous solubility is greatly enhanced for clinical application. The purpose of the present study was to increase the fermentation titer of nocathiacin I and reduce or eliminate analogous impurities by screening the medium ingredients using response surface methodology. After a sysmatic optimization, a water-soluble medium containing quality-controllable components was developed and validated, resulting in an increase in the production of nocathiacin I from 150 to 405.8 mg·L at 150-L scale. Meanwhile, the analogous impurities existed in reported processes were greatly reduced or eliminated. Using optimized medium for fermentation, nocathiacin I with pharmaceutically acceptable quality was easily obtained with a recovery of 67%. In conclusion, the results from the present study offer a practical and efficient fermentation process for the production of nocathiacin I as a therapeutic agent.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Péptidos / Química / Actinobacteria / Reactores Biológicos / Medios de Cultivo / Péptidos y Proteínas de Señalización Intercelular / Fermentación / Mejoramiento de la Calidad / Metabolismo / Antibacterianos Idioma: Inglés Revista: Chinese Journal of Natural Medicines (English Ed.) Año: 2017 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Péptidos / Química / Actinobacteria / Reactores Biológicos / Medios de Cultivo / Péptidos y Proteínas de Señalización Intercelular / Fermentación / Mejoramiento de la Calidad / Metabolismo / Antibacterianos Idioma: Inglés Revista: Chinese Journal of Natural Medicines (English Ed.) Año: 2017 Tipo del documento: Artículo